Optimising kinetic assays to avoid avidity effects

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What you will learn:

  • Researchers from ChromoTek discuss the differences between affinity and avidity as measured on the Octet® BLI platform
  • Measuring affinity in avidity driven assay orientations
  • Why assay orientation, valency, ligand surface density and non-specific binding matter
  • Recognise and resolve issues arising from interactions that contain a bivalent or multivalent component

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