Resources

Assessing the efficacy of vaccines through laboratory assays
In the eighth eBook of the Hamilton series, ‘Assessing the Efficacy of Vaccines through Laboratory Assays’, we discuss: Vaccine development: Key milestones and impact of Covid-19 Laboratory tests commonly used to assess vaccines’ efficacy and the benefits of automating them Hamilton standardised and customised solutions for workflows such as qPCR, NGS, ELISA/ELISpot and virus-neutralisation assays

A comprehensive approach to immunophenotyping for targeted drug discovery and development
With high costs of therapeutic development, success relies on optimising preclinical and early clinical studies. Download this content-rich digital article to read about considerations for implementing and standardising high-dimensional immune profiling methods into drug development programs. Learn about different applications that demonstrate the robustness and reproducibility of a mass cytometry-based analysis to deeply interrogate complex […]

Isomer FOMO? Unlock hidden isomers in your LC-MS analysis
MOBILion Systems is pioneering the evolution of separation science. High-resolution ion mobility (HRIM) on the MOBIE platform offers deeper, more accurate, and efficient characterisation of complex molecules than traditional methods. Download the whitepaper “High-Throughput Monitoring of Biotherapeutic Critical Quality Attributes with High-Resolution Ion Mobility-Mass Spectrometry (HRIM-MS)” to learn more about: Enhancing your analysis of post-translational […]

Advanced flow cytometry handbook
Are you missing out on the easier way to do flow cytometry? In this new handbook, Sartorius introduces the value of advanced flow cytometry, so every scientist feels empowered to run an experiment and analyse the data with confidence. This easy-to-use digital guide includes an introduction to the iQue® Advanced Flow Cytometry Platform and multiplexed […]

Streamline your drug development pathway
In pharmaceutical development, collaborating with multiple Contract Development and Manufacturing Organisations (CDMOs) and Contract Research Organisations (CROs) poses potential hurdles – from poor communication to delays, errors, and inconsistent feedback during the progression from preclinical to formulation optimisation and clinical trials. Download this SGS guide to discover: How to collaborate in the fast-paced world of […]

Stability assessment of biologics during early development workflows
During early development of biologics, there are a large number of attributes researchers evaluate, but some of the most common centre around stability – asking if a biologic is well-folded, active and pure in its buffer, and whether it remains that way over time. Biophysical characterisations are valuable for providing this information without using a […]

Measurement of mitochondrial toxicity using primary hepatocytes
Mitochondrial dysfunction is known to play a central role in drug-induced hepatotoxicity, and thus early detection of mitochondrial toxicity is a critical component of initial drug development efforts. While immortalised cell lines are widely used for in vitro assessment of hepatotoxicity, cryopreserved primary human hepatocytes are a preferred model for drug safety screening. In this App […]

Learn about the rapid bioenergetic functional screening of anticancer drug candidates
The regulation of cellular bioenergetics is recognised as a key driver of cancer cell proliferation. Identifying key pathways that modulate bioenergetic metabolism is a promising strategy for developing novel therapeutics for cancer treatment. In this App Note you will learn: How the Agilent Seahorse XF Real Time ATP rate assay provides a simple robust workflow […]

Optimising kinetic assays to avoid avidity effects
What you will learn: Researchers from ChromoTek discuss the differences between affinity and avidity as measured on the Octet® BLI platform Measuring affinity in avidity driven assay orientations Why assay orientation, valency, ligand surface density and non-specific binding matter Recognise and resolve issues arising from interactions that contain a bivalent or multivalent component

Discover how to achieve mRNA binding on Oligo dT with DBC values over 6mg/mL
In this Application Note, multi-parallel screening of binding conditions on CIM® Oligo dT 96-well plates was performed to optimise the dynamic binding capacity (DBC) in the shortest time possible. What you will learn: How to effectively use Monolithic 96-well plates when developing methods. They are unique on the market in combining the standard well-plate design with monolithic […]

Streamline migration assays with in-incubator monitoring
Cell migration assays can be time-consuming and challenging, from identifying and ensuring assays are imaged at the appropriate time, to properly measuring wound closure. Evident’s CM cell culture monitors provide label-free, quantitative results, consistent results throughout your lab, and are cost-effective. Researchers at ACEL simplified the cell migration process by using Evident’s CM cell culture […]

Analytical methods for viral vector development and manufacturing in gene therapy
In the eleventh eBook of the Hamilton series ‘Analytical Methods for Viral Vector Development and Manufacturing in Gene Therapy’, we learn about: Gene therapy: history, viral vectors and the role of CDMOs Automation of analytical assays during viral vector development and manufacturing The solutions that Hamilton offers to customers working in this field, both in […]

Binding kinetics in hours instead of days with label-free interaction analysis
Label-free interaction analysis takes discovery to a new level. It removes inaccuracies and inefficiencies, but it also uncovers a new layer of insight into the kinetic activity and binding specificity of a greater range of analytes. GCI technology brings you closer to your analytes, allowing you to see interactions in their truest form.

Discover evidence-based, first-in-human dose selection
This whitepaper discusses evidence-based selection of starting doses in first-in-human clinical trials through the utilisation of humanised mouse models and emphasises the importance of accurate dose prediction to ensure patient safety and trial success. In this whitepaper you will learn about: The significance of selecting appropriate starting doses in clinical trials. Utilising humanised mouse models […]

Explore the power of digital PCR for lentiviral vector characterisation
Recombinant lentiviral vectors are used for ex vivo transgene delivery in several gene-modified cell therapies, such as chimeric antigen receptor (CAR) T cell therapy. Reliable analytical methods such as digital PCR to characterise lentiviral vectors and the cells transduced with them are critical to helping ensure the safety and efficacy of these biologics. Download this new […]

The biologics researcher’s mini-guide to screening candidates with nanoDSF
Among the most common critical quality attributes (CQAs) for early developability assessment is thermal stability. One particular tool for assessing thermal stability with high resolution is nano-differential scanning fluorimetry (nanoDSF). Read this mini-guide to learn: How nanoDSF uses your protein’s intrinsic fluorescence to determine its melting temperature, Tm and onset of melting, Ton Why high-resolution […]