Kinetics

‘Optimising kinetic assays to avoid avidity effects’ – a new free resource – is now available from Sartorius and DDW. What you will learn: Researchers from ChromoTek discuss the differences between affinity and avidity as measured on the Octet BLI platform Measuring affinity in avidity driven assay orientations Why assay orientation, valency, ligand surface density […]

What you will learn: Researchers from ChromoTek discuss the differences between affinity and avidity as measured on the Octet® BLI platform Measuring affinity in avidity driven assay orientations Why assay orientation, valency, ligand surface density and non-specific binding matter Recognise and resolve issues arising from interactions that contain a bivalent or multivalent component

Using Promega’s NanoBRET technology, scientists at Aurelia Bioscience have implemented intracellular kinase assays designed to study the interaction of compounds on kinase targets in intact living cells. Advantages of this technique include examination of compound binding to target kinases at physiological ATP concentrations, expression of full-length kinase target in cells, disruption of cells (with digitonin) […]

Aurelia Bioscience is a pre-clinical contract research organisation specialising in bioassay development, pharmacological profiling and compound screening. Scientists at Aurelia Bioscience have experience developing new and innovative bio-assays for different target classes using technologies to deliver client projects in a timely, efficient and cost-effective manner. Using Promega’s NanoBRET technology, Aurelia Bioscience has implemented intracellular kinase […]

Creoptix, a Switzerland-based company focused on next-generation bioanalytical instruments, has launched waveRAPID (Repeated Analyte Pulses of Increasing Duration), a novel, label-free kinetic measurement method that is said to be faster than traditional kinetics. The company developed the Creoptix WAVEsystem to enable molecular interactions to be studied in real time, aimimng to revolutionise drug discovery. By […]