CRISPR screening at scale: Achieving 1m cell data sets with an instrument-free, cost-effective approach

CRISPR-Cas9
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Pooled CRISPR screens offer the ability to knock out or perturb many genes in a single experiment from several hundred to tens of thousands of targets in parallel. Genome-wide screens are becoming the standard for drug discovery to power unbiased identification of drug targets across gene regulatory networks. Requiring single-cell genomics as the data output, these experiments can easily reach millions of cells in a single screen and were previously limited in throughput and scale by both workflow restraints and costs of single-cell profiling.

ScaleBio’s technology enables high-throughput, instrument-free, cost-effective approaches to single-cell omics, and enables highly sensitive capture of guide RNAs matched with gene expression with their CRISPR Guide Enrichment Kit.

In this webinar, Single Cell Discoveries present data on how it was able to use ScaleBio’s technology to perform single-cell RNA sequencing with guide capture on 1M cells from a genome-wide perturbation screen.

An audience Q&A session follows the presentation.

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