Scientists from Korea have developed a new biosensor that uses fluorescence resonance energy transfer (FRET) to detect DNA double-strand breaks in living specimens in real time.
This biosensor has the potential to aid the development of new treatments for DNA damage-related diseases by revealing how our bodies repair damaged DNA.
Double-strand breaks (DSBs) are a type of DNA damage where both strands of DNA break at the same location. They can adversely affect cell growth and functioning.
Currently, DSBs are detected by immunostaining techniques, which identify markers that accompany DNA damage, such as the protein γH2AX. However, these methods are tedious, and cannot be used to detect DSBs in real time in living specimens.
In a 2023 study, researchers describe a fluorescence resonance energy transfer (FRET) biosensor that can detect DSBs in real time and provide time- and location-based information on yH2AX.
The biosensor also has the potential to revolutionise cancer treatment by helping doctors understand how cells respond to treatment and facilitating the discovery of new DNA repair drugs.
“The biosensor we have designed could be useful in areas such as cancer treatment and drug discovery,” says Associate Professor Tae-Jin Kim, from Pusan National University, Korea, who led the study.
FRET sensors consist of two fluorescent proteins or dyes – a donor and an acceptor – which investigate interactions between biological molecules. The energy transfer, and consequently, the amount of emitted light (the FRET signal) depends on the distance and orientation between the two dyes.
“Moreover, as changes in the FRET signal give useful indications of the extent of the DNA damage, the sensor can also be used to examine DNA damage and repair mechanisms, optimise cancer treatments, discover and assess DNA repair drugs, and identify DNA damaging factors in the environment,” concludes Associate Prof Kim.