Join DDW for this exclusive free event, Scaling up in vitro transcription: Key results and considerations, which is supported by Thermo Fisher Scientific.
In vitro transcription (IVT) can be a complicated reaction. Scaling up this process requires a good understanding of the various reaction parameters that need to be optimised and controlled to give the best result in terms of mRNA yields and quality.
In this presentation, a review of the results from scaling up the IVT reaction from μg to gram scale mRNA yield will be presented. The effect of several variables on the IVT reaction using different size DNA templates was assessed. The impact of reaction time, volume, temperature, mixing, reagent concentration, IVT buffer composition and single vs. pulsed addition of reagents on the overall mRNA yield, purity, dsRNA content and capping efficiency will be discussed in detail.
Expert Dr Darwin Asa, Market Development Manager, Thermo Fisher Scientific, will present this event which will take place on 9 November, 4PM GMT, 5PM CET, 11AM EST, 8AM PST.
What you will learn:
- The importance of key reaction variables on the in vitro transcription (IVT) reaction when producing mRNA
- Impacts of changing IVT reaction conditions on mRNA yield, purity, dsRNA synthesis and capping efficiency
- Details of a process to scale up mRNA production to gram scale levels
