Kinetically quantify neuronal outgrowth using live-cell analysis

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Monitoring neuronal morphology in long-term in vitro cell cultures is critical for characterisation and evaluation of disease models and to understand neuronal development.

Ideally, approaches to track neurite dynamics would allow continuous automated measurements of structural parameters, including neurite length and number of branch points. These methods should be non-perturbing and enable quantification of neurons in mono- or co-culture with glia.

This application note describes the use of live-cell imaging to kinetically quantify neurite outgrowth in mono- and co-cultures.

Key learnings:

  • Measuring neuronal parameters in mono-cultures
  • Quantifying neuronal outgrowth in co-cultures
  • Importance of astroglia in iPSC differentiation

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