Indee Labs and GenScript have formed a research collaboration with the aim to develop a DNA knock-in kit for Hydropore to enable large transgene inserts above the 5kb limit for AAV.
As part of the collaboration, GenScript will provide a variety of single-strand and double-strand (ssDNA and dsDNA) constructs for direct DNA inserts, while the team at Indee Labs will optimise the Hydropore protocol and evaluate the functional differences between T cells processed with Hydropore and electroporation.
Looking ahead, the companies will work on co-marketing a complete non-viral solution to the gene and cell therapy community.
“GenScript is pleased to partner with Indee Labs to advance the development of novel RNP and oligo delivery systems for non-viral CRISPR cell engineering,” said Ray Chen, PhD, president of GenScript’s Life Science Group.
The collaboration hopes to empower scientists to insert large transgenes (>5 kb) into immune cells and other cell types via Hydropore delivery of ssDNA and dsDNA constructs.