Bio-Rad Laboratories has launched StarBright Violet 515 (SBV515) Dye.
This is the first of a new range of fluorescent nanoparticles, for use in flow cytometry. StarBright Dyes are conjugated to a growing range of antibodies and are compatible with most flow cytometers and experimental protocols.
The StarBright Violet 515 Dye with an excitation maximum at 401 nanometer and emission maximum of 516 nanometer aims to offersresearchers improved brightness to resolve rare and low antigen density populations more easily. It has a narrow emission profile to reduce spill-over into neighbouring filters and minimal excitation by other lasers, making it suitable for inclusion in multicolour panels.
The dye is not susceptible to photobleaching, delivers high lot-to-lot reproducibility, and is stable at 4°C with no loss of signal to help ensure consistent staining and deliver reliable results. It has been tested in many common staining buffers and there is no requirement to use a special staining buffer, permitting easy integration into common experimental protocols without compromising performance. StarBright Violet 515 Dye is compatible with Bio-Rad’s ZE5 Cell Analyzer, S3e Cell Sorter, as well as other cytometers, and it is suitable for both conventional and spectral flow.
“StarBright Violet 515 Dye is brighter than other common dyes that emit at similar wavelengths,” said Mike Blundell, Bio-Rad Product Manager, Life Science Group. “The dye is flexible enough to fit into any flow cytometry panel, allowing researchers to multiplex with ease regardless of flow cytometer and experimental protocol, and it offers a superior alternative for fluorophores that have emission maxima between 500 and 550 nanometers.”
Image: Daniele Levis Pelusi