A free webinar, Sensitive signature peptide quantification using accurate mass spectrometry, was hosted by DDW on December 1, 2021, at 4PM GMT, 9AM PT; 12PM ET; 5PM CET. It was supported by SCIEX.
Emerging advancements in protein biotherapeutics are driving the need for more sensitive and accurate bioanalytical methods for their quantification. Serving as an orthogonal technology to the traditional ligand binding assays (LBAs), LC-MS has been routinely adopted for quantitative measurement of protein levels in bioanalytical laboratories.
To improve the sensitivity issues related to the detection of relatively large biotherapeutic peptides, researchers can quantify signature peptides—small, unique peptides resulting from tryptic digests of the parent protein—rather than directly detect the intact protein itself. This technique can lead to a significant improvement in sensitivity, achieving LLOQs as low as 10 pg/mL. However, during bioanalysis, overlapping peaks or impurities from biological matrices can negatively affect sensitivity and selectivity. In these cases, relying on MRM transitions alone for peptide detection may be inadequate for overcoming the isobaric interferences and high baseline noise that can negatively influence the signal-to-noise ratios during bioanalysis.
Dr. Xin Zhang, Senior Application Scientist at SCIEX, presented an assay development method using accurate mass spectrometry to quantify a series of signature peptides in rat plasma. She also provided an overview of the fundamental steps to develop a highly sensitive and selective quantitative assay for signature peptides.
This webinar will benefit any scientist and lab director involved in bioanalytical assay development using LC-MS/MS.
Watch on demand for free here.