Mirus Bio outlines when to use transfection reagents, viral transduction or electroporation.
No single delivery method is ideal for all situations, but researchers may routinely employ a suboptimal approach for the sake of familiarity or to avoid any start-up costs associated with new methods.
The uses of nucleic acid delivery have expanded from recombinant gene expression to a diversity of other experimental applications, including gene knockdown with small interfering RNAs (siRNA) and genome editing with CRISPR ribonuclear protein complexes (RNPs).
With these advances, three approaches have become a mainstay for delivering nucleic acid cargo into mammalian and insect cells: chemical transfection, viral transduction, and electroporation. This listicle highlights and when to use, the benefits of each approach.
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