Selecting the best gene delivery method

Cell and gene therapy

Mirus Bio outlines when to use transfection reagents, viral transduction or electroporation.

No single delivery method is ideal for all situations, but researchers may routinely employ a suboptimal approach for the sake of familiarity or to avoid any start-up costs associated with new methods.

The uses of nucleic acid delivery have expanded from recombinant gene expression to a diversity of other experimental applications, including gene knockdown with small interfering RNAs (siRNA) and genome editing with CRISPR ribonuclear protein complexes (RNPs).

With these advances, three approaches have become a mainstay for delivering nucleic acid cargo into mammalian and insect cells: chemical transfection, viral transduction, and electroporation. This listicle highlights and when to use, the benefits of each approach.

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