Reorganisation of the tumour microenvironment in response to KRASG12C inhibition in a model of lung adenocarcinoma

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This FREE TO REGISTER webinar includes three presentations.

Title 1: Resolving complex biology with single cell and spatial multiomics Single cell and spatial tools from 10x Genomics deliver multiomic insights from complex disease samples to fuel the drug discovery pipeline with promising candidates. See how these tools help understanding of disease mechanisms, accelerating novel therapeutics discovery.

Title 2: TotalSeq reagents for single cell Multiomics: Uncover unique phenotypes and understand protein and RNA expression on a single-cell level with TotalSeq oligo-conjugated antibodies Advances in scRNA-seq allow researchers to analyse cell surface immunophenotype and transcriptome simultaneously. BioLegend offers TotalSeq oligo-conjugated antibodies that integrate with existing scRNA-seq protocols. TotalSeq reagents enable multiplexed measurement of cell surface molecules for novel applications in precision medicine, oncology, immunology, neuroscience and stem cell research.

Title 3: Reorganisation of the tumour microenvironment in response to KRASG12C inhibition in a model of lung adenocarcinoma The clinical approval of sotorasib, the first drug targeting KRASG12C, was a milestone in lung cancer treatment. However, resistance occurs rapidly and most patients relapse. KRASG12C inhibitors induce changes in the expression of genes involved in the modulation of the tumour microenvironment, suggesting an opportunity to tackle the development of resistance by combining these drugs with immunotherapy. To understand more, an orthotopic mouse model of lung cancer was used carrying a KRASG12C mutation, the 3LL cell line, and treated 3LL-tumour-bearing mice with MRTX1257. CITE-Seq was performed on these tumours, the changes in immune cell representation and the differential gene expression occurring in the immune cells after treatment were measured. It was observed that the profound remodelling of the macrophage compartment after treatment was consistent with lab findings using imaging mass cytometry.

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