White Papers (Old)
The Effect of Amplicon Charcteristics on the success of Fast QPCR
By: Gerwyn Jones, Sryana Kapavaparu, Saima Nayab and Ian Kavanagh
ThermoFisher Scientific

Employing fast QPCR cycling protocols is a simple and effective way of maximising throughput by reducing run durations. Fast cycling protocols can easily be achieved by reducing the temperature step dwell times.

It has been reported that the use of fast QPCR protocols can result in a loss of sensitivity and greater inter-replicate variance with some assays. It is therefore important to realise that speeding up cycling protocols can be detrimental to the quality of results, but not in all cases. The factors which determine whether an assay can be successfully employed using fast cycling conditions are poorly understood.....

To see the full poster please download the PowerPoint file on the right.