Scaffold-free 3D culture systems that generate spheroids (and other similar multicellular aggregations) have proved useful as they offer an easy route to access 3D culture and transition into plate-based higher throughput. The emergence of ultra-low attachment (ULA) or cell-repellent plates that generate spheroids has been a major factor in increasing investigation and adoption of 3D culture. The popularity of ULA plates for spheroid generation has shifted the emphasis in spheroid generation ..........

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