Thermo Fisher Scientific Inc. and SRI International, have announced the results of a collaboration agreement to enable researchers to combine the results of high-resolution Orbitrap LC/MS experiments with highly curated and organism-specific metabolic pathway and genome data for quick and effective mass spectrometry-based small molecule research and analysis.
Messenger RNA (mRNA), the naturally occurring molecule honed over thousands of years of evolution to translate accurately and efficiently the information encoded in a cell's DNA into the proteins essential for maintaining cell function and ensuring an organismâ€™s survival, will revolutionise the biopharmaceutical industry.
A decade and a half after being first described to occur in mammalian cells, numerous trials are indicating that RNA interference can be harnessed to treat human disease. This article argues that positive results are now starting to emerge for the application of vector-based ddRNAi.
Despite an investment of billions of US dollars in the search of novel therapies, cancer still remains the leading cause of death in the world. This emphasises the need to identify novel tumour dependencies and molecular targets.
RNAi screening is arguably the fastest growing field with the premise to better understand gene function at the genome level. It has been hailed as the second genomics wave, and in combination with the human genome-sequencing projects, would constitute the holy grail of modern genetics.
Even in the age of genomics and gene hunters, a holistic view of cell metabolism can result in revolutionary new drugs.
In the post genomic era, elucidation of the physiological function of genes has become a major rate-limiting step in the quest to develop ‘gene-based-drugs’. As we advance in the ‘functional omics’ arena with a hope to discover novel drug targets and therapies, their validation is a pivotal step before clinical practice. Such an endeavour can be tested using small interference RNAs (siRNAs) or RNA-mediated genetic interference (RNAi).
Libraries of siRNAs directed against predefined subsets of genes now offer the capacity to greatly accelerate and improve the quality of functional genomics based drug discovery by enabling a much more targeted approach that effectively integrates the discovery and validation of novel targets.
One of the major challenges facing the pharmaceutical industry today is the identification and validation of novel, high-quality drugable targets in underserved disease areas.This paper briefly outlines the latest advances in in vitro siRNA-based screening of gene function for target discovery.Additionally, the identification and validation of targets for osteoarthritis therapies through the functional screening of adenoviral siRNA libraries in human primary chondrocytes will be presented as a case study for this technology. Using this approach, both new targets and previously known regulators of chondrogenesis were identified and validated.This functional screening technique has also been applied to the discovery of novel, drugable targets for therapeutic development in Alzheimer’s disease, asthma, osteoporosis and rheumatoid arthritis.
RNAi (RNA interference) is an important target validation tool that has risen to prominence since 2001. For a number of reasons, it has supplanted other pre-existing target validation tools and is now the method of choice for target validation in cell culture systems.
Purified RNA is crucial for use in a range of downstream applications performed by life scientists, most notably reverse transcription PCR (RT-PCR), quantitative real-time PCR (qPCR) and cDNA synthesis. RNA purification is an area of particular salience for life science suppliers, as the successful extraction of RNA poses not only technical challenges to researchers, but also commercial challenges of a heterogeneous marketplace due to variation in end users’ sample sources, level of throughput and downstream applications.
In this article, a recent market analysis is discussed aimed at understanding the market landscape, trends and opportunity in the rapidly-expanding microRNA marketplace. These market analyses are presented in detail in a recently published MicroRNA 2008 market report.