Biotrove makes life alot easier with RapidFire

Use of RapidFire technology requires no labels or surrogate substrates -- the system enables detection of native compounds without the need for fluorescence, luminescence or radioactivity. The result is in vitro ADME without compromises.  The RapidFire System integrates fast, serial, on-line sample purification with mass spectrometric analysis.

With sample cycle times of six seconds per sample, this system can obtain a 7 point IC50 curve in just 42 seconds -- less time than a single sample using standard HPLC methods. The system integrates with any manufacturer’s triple quadrupole mass spectrometer and provides output data in plate reader format that is compatible with existing laboratory information management systems.

The RapidFire 300 is capable of analyzing most in vitro ADME assays without modification to existing methods or workflows. In vitro ADME applications such as CYP inhibition, metabolic stability, P-glycoprotein inhibition, plasma protein binding, permeability (Caco-2 and PAMPA) and CYP induction can be performed using the RapidFire 300 System. Multiple MS analysis methods can be run with a single setup and unattended 24 hour operation is possible.

Data from a variety of in vitro ADME assays shows that results from the RapidFire system are comparable to those from traditional LC-MS systems. The RapidFire 300 system increases the efficiency of in vitro ADME drug discovery workflows providing high quality data in a fraction of the time required for conventional HPLC techniques.  For more information on the RapidFire 300 and our other products and services, please visit www.biotrove.com.