A highly reproducible cell migration screening assay...

The Oris™ Cell Migration Assay provides life scientists with a method that is more reliable, less labour intensive, allows for real-time imaging, and is amenable to high throughput and high content imaging instruments. Unlike traditional cell migration assays using a Boyden chamber device, there is no transmembrane or cell culture insert obstructing live images of the cells as they migrate.

Described in a new video (www.amsbio.com/videos-Oris-Cell-Migration-Assay-Video.aspx) the Oris™ Cell Migration Assay uses a multistep process that starts with the attachment (optional) of a detection mask to the bottom of the wells of the Oris™ 96-well assay microplate. A specially designed, cell seeding stopper fitted inside of each microplate well restricts cells from seeding into a central detection zone as cells are being dispensed. After cell attachment has occurred (4-28 hrs), the stoppers are removed, allowing cells to freely migrate into the detection zone of the wells. Cells can be labeled pre- or post-migration.  Cells that have not migrated into the detection zone are blocked from view by the mask initially applied to the plate bottom. Migrated cells may then be analysed using a fluorescence plate reader. If analyzing the migrated cells using a microscope or high content imaging system, the detection mask need not be attached. Oris™ Cell Migration assays are available with tissue culture treated plates, Collagen I coated plates, Fibronectin coated plates, or a TriCoated version that contains all 3 surfaces.

Wound healing assays are normally performed using a scratch assay, which has high variability. The Oris™ Cell Migration Assay is an ideal alternative to the scratch assay since the cell seeding stoppers create a consistent detection zone to monitor 2-D closure allowing reproducible data to be collected. The Oris Assay gives researchers the option to take real time, video images of cell movement or to quantify cell migration using a fluorescent microplate reader.   The Oris Cell Migration Assay format allows researchers to capture more data per well using a number of instrument platforms to monitor cell movement.

Founded in 1987, AMS Biotechnology (amsbio) is recognised as a leading international provider of unique & innovative products & custom services for life sciences research. The amsbio range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signaling, DNA damage, electrophoresis, glycobiology, posttranslational modification & stem cell biology.

For further information on Oris Cell Migration Assay kits please contact amsbio on telephone +44-1235-828200 or email PhillipP@amsbio.com


**  Cell migration is a fundamental activity intrinsic to development and maintenance of homeostasis in processes such as wound healing, neovascularisation and the workings of the immune system. The failure of cells to migrate, or the movement of invasive cells into inappropriate locations, is central to many disease processes such as dysregulated wound healing and cancer. Additionally, understanding cell migration is critical to emerging technologies such as tissue engineering and the successful bio-integration of prosthetic devices.
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