The ROX-blended version of the SsoFast EvaGreen supermix is optimized for users of PCR systems that employ ROX as a passive reference for normalization. SsoFast EvaGreen supermix with low ROX can be used effectively on Bio-Rad's real-time PCR detection systems as well as on the ABI 7500 Fast and Standard, ABI StepOne and StepOnePlus, and Stratagene MX-series real-time PCR systems.


"By pre-blending ROX into the SsoFast EvaGreen supermix, ABI 7500 users can experience the same performance advantages achieved with our SsoFast EvaGreen supermix," said Viresh Patel, Bio-Rad Senior Product Manager, PCR Reagents.


Through instant polymerase activation, optimal primer binding, and rapid polymerization kinetics, SsoFast EvaGreen supermix with low ROX can help researchers reduce their time-to-results from 90 minutes or more for standard cycling to between 35 and 45 minutes. Under these fast qPCR conditions, real-time PCR experiments maintain broad dynamic range, reliability, and accurate quantification.


The patented Sso7d-fusion polymerase utilizes the dsDNA-binding protein Sso7d to enable fast cycling without affecting PCR sensitivity, efficiency, or reproducibility. In addition, the Sso7d-fusion polymerase is significantly more resistant to PCR inhibitors, making SsoFast EvaGreen supermix with low ROX an ideal choice for applications such as qPCR directly from unpurified sample (direct PCR).


For more information about Bio-Rad's family of SsoFast PCR reagents, visit www.bio-rad.com/supermixes.


About Bio-Rad


Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), has remained at the center of scientific discovery for more than 50 years, manufacturing and distributing a broad range of products for the life science research and clinical diagnostic markets. The company is renowned worldwide among hospitals, universities, major research institutions, as well as biotechnology and pharmaceutical companies for its commitment to quality and customer service. Founded in 1952, Bio-Rad is headquartered in Hercules, California, and serves more than 85,000 research and industry customers worldwide through its global network of operations. The company employs over 6,800 people globally and had revenues of nearly $1.8 billion in 2009. For more information, visit www.bio-rad.com.