Insulin is a peptide hormone made in the pancreas by beta cells and promotes the uptake of glucose from the blood into tissues, where it is stored in the form of glycogen and fat. By controlling glucose levels, insulin serves as the central regulator of fat and carbohydrate metabolism.

Proinsulin is composed of the A and B subunits of insulin and is connected by the C-peptide region. Proinsulin is processed in the endoplasmic reticulum of pancreatic b-cells and is converted within immature secretory granules into insulin and C-peptide. While mature insulin is required for glucose homeostasis and C-peptide is associated with renal function, proinsulin has low metabolic activity. The release of proinsulin is significantly increased in patients with type 2 diabetes mellitus, as well as, in individuals with impaired glucose tolerance

The Insulin and Proinsulin ELISA kits allow for the quantitative determination of insulin and proinsulin in serum and plasma.  These ELISA kits provide a highly sensitive measurement, detecting as little as 0.622 µIU/mL (insulin) and 0.17 pM (proinsulin), providing fully quantitative results that surpass semi-quantitative Western blot analysis. With the ability to analyze up to 40 samples in duplicate in 3 hours, these kits meet the needs for rapid, high-throughput analysis.

Like many other Enzo Life Sciences ELISA kits, these kits are complete, flexible and thoroughly validated. Customers are supplied with a simple protocol, a pre-coated microtiter plate and liquid color-coded reagents to save time and reduce errors. The break-a-part strip plate design provides flexibility – the user does not have to run all 96-wells at once. Each kit is taken through rigorous fit-for-purpose validation and stability testing to ensure accuracy, sensitivity, and specificity.

For more information or to order the Proinsulin and Insulin ELISA kits or other Enzo Life Sciences products, please visit us at or call us at 1-800-942-0430.