The SYNAPT G2-S incorporates both high-sensitivity Waters StepWave™ ion transfer 
optics and Triwave® ion mobility technologies along with a suite of new
informatics tools to take qualitative and quantitative high resolution
performance to a new level.

SYNAPT G2-S provides a 30X improvement in signal intensity for mass spectral
peaks, better than a 5X improvement in signal-to-noise, and up to a 10-fold
improvement in limits of quantitation over previous-generation mass
spectrometers. The significant increase in sensitivity, together with the
unrivalled selectivity and analytical peak capacity made possible by ion
mobility separations, allows the SYNAPT G2-S to identify and quantify sample
analytes at lower concentrations than any other high resolution mass
spectrometer and easily and routinely reveal details about complex samples
that were previously difficult or impossible to obtain. Introduced at the
59th ASMS Conference on Mass Spectrometry, the SYNAPT G2-S is expected to
begin shipping at the end of September.

StepWave ion-transfer optics technology employs a revolutionary off-axis
design for dramatically increasing the efficiency of ion transfer from the
ion source to the MS analyzer, while at the same time actively eliminating
undesirable neutral contaminants. The resulting sensitivity improvement is
critical, for example, in metabolite identification where the goal is to
find, identify and quantify all metabolites in a sample. Experiments like
these can be performed for the first time at biologically relevant
concentration levels which give the chemist a much more accurate
quantitative and qualitative understanding of the molecular system of
interest to better aid the drug development processes.

“When research scientists set out on a mission of discovery, they want to
know everything there is to know about their analyte – what it is, how much
is present, how it’s been modified and even what its conformation is like.
Ion mobility and the UPLC/MSE data-independent acquisition mode of the
SYNAPT G2-S give scientists unrivalled analytical selectivity for the most
confident and comprehensive characterization of complex analytes and
mixtures of complex compounds, and unprecedented sensitivity to make this
possible at lower concentrations than on any other high resolution mass
spectrometry systems,” says Alistair Wallace, Ph.D., SYNAPT Product Manager,
Waters Division. “Furthermore, it complements NMR, electron microscopy, and
x-ray crystallography and gives scientists a unique opportunity to
selectively and accurately study the shape of molecules which are beyond the
reach of these traditional techniques.”

Waters SYNAPT G2-S supports Waters' exclusive High-Definition MSE
acquisition method which comprehensively catalogs complex samples in a
single analysis by separating components by ion mobility and then capturing
the molecular and fragment ion data from all detectable peaks across the
entire chromatographic separation, all in a single injection. The ability of
ion mobility to separate isobaric/isomeric compounds from one another is the
key to reaping the benefits of the sensitivity enhancements of StepWave,
dramatically increasing the number of confidently identified components. The
result is accurate qualitative and quantitative profiling of all of the
components over a linear dynamic range of up to five orders of magnitude
aided by the high resolution quantitative time-of-flight (QuanTofTM)
analyzer featuring an ultra-fast electron multiplier and hybrid
analog-to-digital (ADC) detector electronics. The instrument’s mass
resolving power is over 40,000 FWHM (full width half maximum).

SYNAPT G2-S also includes additional software to provide unrivalled
experimental versatility to meet a wide range of challenges:

- MSE data viewer for visualization, processing, and interpretation of
multidimensional MS or HDMS data;

- High Definition Imaging Software for leveraging the ability of ion
mobility separations to maximize selectivity and confidence in MALDI imaging

- Dedicated proteomics and biopharmaceutical software application managers
that fully support HDMSE data processing and reporting.

- Improved ease-of-use features allowing scientists to take advantage of
SYNAPT’s unique Time-Aligned-Parallel fragmentation capability and the
powerful Electron Transfer Dissociation (ETD) capability - providing
faster, more complete and confident structural identification and
characterisation of target analytes.

- Combining faster spectral acquisition rates of up to 30 spectra/sec with
new embedded algorithms to optimize data-dependent analyses (DDA) to
efficiently deliver the highest quality MS/MS spectra for compound
identification or protein quantification by isotope labeling.

The SYNAPT G2-S also features Waters Universal Ion Source Architecture
engineered to take maximum advantage of UPLC® allowing the widest range of
Waters ionization sources including: electrospray (ESI), atmospheric
pressure chemical ionization (APCI), dual ESI and APCi (ASCi), atmospheric
pressure photoionization (APPI), atmospheric pressure gas chromatography
(APGC), NanoFlow® (ESI), matrix assisted laser desorption (MALDI),
atmospheric solids analysis probe (ASAP) and TRIZAIC UPLC®. It is also
compatible with third party ionization sources including DESI (Prosalia),
DART (IonSense), LDTD (Phytronix), and the TriVersa nano Mate (Advion)

Primary applications for the SYNAPT G2-S include proteomics, biomarker
discovery, pharmaceuticals/biopharmaceuticals, lipidomics, structural
elucidation, metabolomics, polymer analysis, petroleum characterization,
metabolite identification and food research.

Since its introduction in 2006, SYNAPT HDMS has set a new standard in
high-end MS instrumentation. To date there have been more than 150 research
papers published to date based on Triwave ion mobility SYNAPT technology and
more than 70 research papers on UPLC/MS(E).

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