REAL-TIME PCR - where we are and where are we heading

By Dr Mikael Kubista

Quantitative real-time PCR is becoming mature technology for the quantification of nucleic acids. It is spreading wide outside its original use in the research laboratories, becoming preferred technology for a range of applications, many that require specialised solutions and adaptations. Integration with pre-analytical steps and post-processing operations are becoming key challenges. more...

COMPOUND PROFILING size impact on primary screening libraries

By William Downey, Cindy Liu and Dr Jennifer Hartigan

A recent report examines the trends in utilisation of compound libraries, as well as the impact of profiling on the size of screening decks. The drivers for choosing between large or focused libraries may be different, but the ultimate goal remains the same: improve drug discovery. more...

The Microplate Market Past, Present and Future

By Dr Peter Banks

Microplates and the peripheral markets of liquid handling and detection technologies exist within almost all laboratories performing assays. This article discusses what, if any, are their growth expectations and whether there are new technologies on the horizon that could make the microplate obsolete. more...

Letting the Target Determine your Compound Acquisition Strategy

By Dr John Harris

With compound libraries likely to remain the cornerstone of hit and lead discovery for the foreseeable future, this paper seeks to summarise current and future approaches to optimal library design and compound acquisition, using examples and experimental evidence of the success, limitations and weaknesses of target-led approaches in comparison with diversity-screening alternatives. more...

Monochromator vs Filter-based Plate Readers; horses for courses, or a winning combination?

By Dr John Comley

This article is based around feedback obtained in HTStec’s recent Multi-Mode Microplate Reader Trends 2007 Report. more...

qPCR Assays; end user practices and latest vendor offerings

By Dr John Comley

This review provides an insight into real-time quantitative PCR (qPCR) assays today. more...