Removal of Contaminating Genomic DNA in QRT-PCR using a Shrimp Nuclease

Nicky Quipse, Saima Naveed Nayab and Ian Kavanagh

DNA contamination can often occur in quantitative reverse transcription – polymerase chain reactions (QRT-PCR), and should be removed in order to avoid false positive results. more...

The Effect of Amplicon Charcteristics on the success of Fast QPCR

Gerwyn Jones, Sryana Kapavaparu, Saima Nayab and Ian Kavanagh

Employing fast QPCR cycling protocols is a simple and effective way of maximising throughput by reducing run durations. Fast cycling protocols can easily be achieved by reducing the temperature step dwell times. more...

The Effect of White Pigmentation in 96 Well Plates during QPCR

Saima N. Nayab, Philip N.Harries, Simon C. Baker and Ian Kavanagh

QPCR instruments are able to monitor amplicon quantity in real-time during PCR reactions by detecting fluorescence signals and recording fluorescence data. more...